Tip: Emphasize that the 300 bp doublet runs as one thicker band.
Describe or sketch band positions, noting any comigrating bands (different fragments same size). Lab Dna Restriction Enzyme Simulation Answer Key
In the triple digest (EcoRI, HindIII, BamHI), if you ran the gel and saw four bands instead of three, what could explain this? A (key): Incomplete digestion (one site remains uncut), leading to partial products that sum to longer fragments. Tip: Emphasize that the 300 bp doublet runs